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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC18.17c
Gene ID 23/F04
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1467
ORF length (spliced)
Entry clone length 1467
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC18.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATAGTATAGAACTCAT
Rev primer name SPCC18.17.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAGTTTCTGCGGCCACT
Amino acid length 488
Molecular weight 55.3
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQINFVDVLGI
Localization (YFP) nucleus>cytosol; nuclear envelope
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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