Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31A2.01
Gene ID 23/C02
Gene synonyms/obsolete csn71; csn7b; csn7; csn7-2; SPAC1751.03
Gene product COP9/signalosome complex (subunit 7b); PCI domain; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1443
ORF length (spliced) 1209
Entry clone length 1443
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31A2.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGGCTCTGATTTTAT
Rev primer name SPAC31A2.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTCAGAGGCTGTGACCGCG
Amino acid length 402
Molecular weight 45
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNLFLTRLPL/LTPAILSVLSI
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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