Gene name |
SPCC576.06c |
Gene ID |
23/B09 |
Gene synonyms/obsolete |
|
Gene product |
tyrosine-tRNA ligase;
involved intyrosyl-tRNA aminoacylation; tyrosine-tRNA ligase
activity |
Entry clone |
Cloned |
ORF length (unspliced) |
1436 |
ORF length (spliced) |
1338 |
Entry clone length |
1436 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC576.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCACGTTTACTAGCATG |
Rev primer name |
SPCC576.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGAGAGTCAAGTACAAAA |
Amino acid length |
445 |
Molecular weight |
49.4 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDMLTLLPL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |