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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC576.06c
Gene ID 23/B09
Gene synonyms/obsolete
Gene product tyrosine-tRNA ligase; involved intyrosyl-tRNA aminoacylation; tyrosine-tRNA ligase activity
Entry clone Cloned
ORF length (unspliced) 1436
ORF length (spliced) 1338
Entry clone length 1436
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC576.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCACGTTTACTAGCATG
Rev primer name SPCC576.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGAGAGTCAAGTACAAAA
Amino acid length 445
Molecular weight 49.4
Isoelectric point (calc.) 9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDMLTLLPL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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