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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC630.07c
Gene ID 22/D09
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1293
ORF length (spliced)
Entry clone length 1293
No. of intron 0
Sequence status Finished
Sequence results 32A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC630.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATCAATATCGTGATTT
Rev primer name SPAC630.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATCGTATGTAATTAGGTCA
Amino acid length 430
Molecular weight 48.7
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTSMFVPLWL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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