| Gene name |
SPBC3E7.11c |
| Gene ID |
22/C03 |
| Gene synonyms/obsolete |
|
| Gene product |
DNAJ domain protein;
similar to Sp SPAC4H3.01 (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1237 |
| ORF length (spliced) |
1068 |
| Entry clone length |
1237 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
15T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3E7.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTCAAAGAGTTGTCGA |
| Rev primer name |
SPBC3E7.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTACCTCTGTCGTTCATT |
| Amino acid length |
355 |
| Molecular weight |
40.5 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCKNLLDKLSI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
Confocal |