Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC26F1.03
Gene ID 22/C01
Gene synonyms/obsolete pda1
Gene product pyruvate dehydrogenase e1 component (alpha subunit); involved in acetyl-CoA biosynthesis from pyruvate; pyruvate dehydrogenase (lipoamide) activity
Entry clone Cloned
ORF length (unspliced) 1230
ORF length (spliced)
Entry clone length 1230
No. of intron 0
Sequence status Finished
Sequence results 1028A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC26F1.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTCGAACTTGTACGAA
Rev primer name SPAC26F1.03.Rv
Rev primer SEQ AGAAAGCTGGGTACTTATATTGATGATATTCC
Amino acid length 409
Molecular weight 45.1
Isoelectric point (calc.) 8.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.