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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC126.06
Gene ID 20/F05
Gene synonyms/obsolete twf1
Gene product twinfilin; actin cortical patch component; actin-binding protein; ADF family; cofilin/tropomyosin-type; putative actin monomer sequestering protein involved in regulation of the cortical actin cytoskeleton; possibly prevents actin filament assembly; involved in actin cortical patch distribution (pers. comm. David R. Kovar and Thomas D. Pollard); non-essential (pers. comm. David R. Kovar and Thomas D. Pollard)
Entry clone Cloned
ORF length (unspliced) 1333
ORF length (spliced) 987
Entry clone length 1333
No. of intron 6
Sequence status Finished
Sequence results 961G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC126.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGCTTCCGTCGAATT
Rev primer name SPCC126.06.Rv
Rev primer SEQ AGAAAGCTGGGTAACGTCTTCTAGGGGGTCGA
Amino acid length 328
Molecular weight 36.7
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLDSIKAELGI
Localization (YFP) cytoplasmic dots at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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