Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC16.05c
Gene ID 20/E11
Gene synonyms/obsolete
Gene product zinc finger protein; zf-C2H2 type (2)
Entry clone Cloned
ORF length (unspliced) 1329
ORF length (spliced)
Entry clone length 1329
No. of intron 0
Sequence status Finished
Sequence results 121A:G / 519T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC16.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCATCTCTCGCATTGCC
Rev primer name SPAC16.05.Rv
Rev primer SEQ AGAAAGCTGGGTATGCGGTGCGCATAAAGTTC
Amino acid length 442
Molecular weight 48.2
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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