Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1198.01
Gene ID 20/D04
Gene synonyms/obsolete
Gene product glutathione-dependent formaldehyde dehydrogenase; no apparent Sc ortholog; conserved in microbes
Entry clone Cloned
ORF length (unspliced) 1272
ORF length (spliced)
Entry clone length 1272
No. of intron 0
Sequence status Finished
Sequence results 932A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1198.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAATATCTAACCAATTT
Rev primer name SPBC1198.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTCCGTCATATCACAATAT
Amino acid length 423
Molecular weight 46.4
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi
Comments for localization accumulated Golgi by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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