| Gene name |
SPAC6G10.06 |
| Gene ID |
20/B12 |
| Gene synonyms/obsolete |
|
| Gene product |
amino acid oxidase;
similar to Sp SPAC1F5.03C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1264 |
| ORF length (spliced) |
1131 |
| Entry clone length |
1264 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
214A:G / 476A:deletion
/ 1235G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6G10.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCGCTACAAGTAGAGA |
| Rev primer name |
SPAC6G10.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCACCGAGATTTAAGTGA |
| Amino acid length |
376 |
| Molecular weight |
41.7 |
| Isoelectric point (calc.) |
8.2 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNTMGNLNI/LKEMTDLVL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |