| Gene name |
SPACUNK12.02c |
| Gene ID |
20/A06 |
| Gene synonyms/obsolete |
cmk1 |
| Gene product |
serine/threonine
protein kinase; calmodulin kinase I homolog; CaM-dependent
protein kinase; cell cycle-dependent expression; predicted
regulatory site (Thr-192) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1256 |
| ORF length (spliced) |
1008 |
| Entry clone length |
1256 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
37A:G / 246A:G /
465T:C / 1234A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPACUNK12.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGCAAACATACAAACC |
| Rev primer name |
SPACUNK12.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGCTTATTCTCAAGCTTT |
| Amino acid length |
335 |
| Molecular weight |
38.1 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |