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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC215.09c
Gene ID 19/H09
Gene synonyms/obsolete
Gene product acetyl-coA C-acetyltransferase activity; involved in ergosterol biosynthesis
Entry clone Cloned
ORF length (unspliced) 1248
ORF length (spliced) 1188
Entry clone length 1248
No. of intron 1
Sequence status Finished
Sequence results 166A:G / 873T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC215.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTAACACGTATGTGAT
Rev primer name SPBC215.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTCTTTCGATAACAATG
Amino acid length 395
Molecular weight 40.9
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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