| Gene name |
SPBC582.07c |
| Gene ID |
19/E12 |
| Gene synonyms/obsolete |
rpn7 |
| Gene product |
19S proteasome
regulatory subunit; essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1230 |
| ORF length (spliced) |
|
| Entry clone length |
1230 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
91G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC582.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGTTCTCCCTCTGCAAC |
| Rev primer name |
SPBC582.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACCTTAAAGGCACCACGC |
| Amino acid length |
409 |
| Molecular weight |
46.6 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEACINSLYL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |