| Gene name |
SPAC1D4.02c |
| Gene ID |
19/E09 |
| Gene synonyms/obsolete |
|
| Gene product |
GRASP55/65 family;
mammalian GRASP protein homolog predicted; putative golgi
stacking machinery; spindle checkpoint protein; similar to
S. cerevisiae YDR517W |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1229 |
| ORF length (spliced) |
1038 |
| Entry clone length |
1229 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1D4.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGGTGGTTTAAAGAA |
| Rev primer name |
SPAC1D4.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTTCACAAGTTCTTCA |
| Amino acid length |
345 |
| Molecular weight |
37.8 |
| Isoelectric point (calc.) |
5.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots at
cell tip and site of septum formation; cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |