Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC660.08
Gene ID 18/H09
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; predicted coiled-coil region
Entry clone Cloned
ORF length (unspliced) 1188
ORF length (spliced)
Entry clone length 1188
No. of intron 0
Sequence status Finished
Sequence results 772T:C/ 1159A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC660.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTATTATGGATTAGCAAA
Rev primer name SPBC660.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGAAGATCTTGATCGAGA
Amino acid length 395
Molecular weight 45.1
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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