Gene name |
SPAC3G6.03c |
Gene ID |
18/G08 |
Gene synonyms/obsolete |
|
Gene product |
Maf-like protein;
involved in cytokinesis; involved in septum formation
(regulation) (negative); involved in septation |
Entry clone |
Cloned |
ORF length (unspliced) |
1184 |
ORF length (spliced) |
711 |
Entry clone length |
1184 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
790T:C / 828G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3G6.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATACGTCTCCTCCTTC |
Rev primer name |
SPAC3G6.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCGTAATTATCAGCATCT |
Amino acid length |
236 |
Molecular weight |
26.3 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|