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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3D6.02
Gene ID 18/E12
Gene synonyms/obsolete
Gene product Sp specific families; glycoprotein; similar to Sp SPAC27D7.09c and SPAC27D7.10c and SPAC27D7.11c (paralogs)
Entry clone Cloned
ORF length (unspliced) 1173
ORF length (spliced)
Entry clone length 1173
No. of intron 0
Sequence status Finished
Sequence results 6A:T / 340A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3D6.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAATTGTTAAACTCTTT
Rev primer name SPBC3D6.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATCGAAGTTAGCATTGGAG
Amino acid length 390
Molecular weight 43.6
Isoelectric point (calc.) 9.2
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

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