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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23C4.08
Gene ID 18/D04
Gene synonyms/obsolete rho3
Gene product Rho family; small GTPase; involved in cellular morphogenesis; involved in septation; involved in microtubule cytoskeleton organization and biogenesis (IMP); involved in actin cytoskeleton organization and biogenesis; involved in regulation of the actin cytoskeleton; involved in exocytosis (IMP); interacts physically with Myo2p; interacts physically with Exo70p; involved in cell polarity; interacts physically with For3p; C-terminal CAAX motif; involved in cytokinesis; involved in cytokinesis, cell separation (regulation)
Entry clone Cloned
ORF length (unspliced) 1164
ORF length (spliced) 627
Entry clone length 1164
No. of intron 3
Sequence status Finished
Sequence results 179C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23C4.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCTTCGCTAAGCAGTA
Rev primer name SPAC23C4.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAATGATACATCCGGTA
Amino acid length 208
Molecular weight 22.8
Isoelectric point (calc.) 5.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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