Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H4.09
Gene ID 18/B01
Gene synonyms/obsolete cdb4
Gene product curved DNA-binding protein??; metallopeptidase; peptidase family M24; similar to human and M. musculus P38-2G4 cell cycle protein; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1146
ORF length (spliced)
Entry clone length 1146
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H4.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGACTAAAGAAGCAAC
Rev primer name SPAC23H4.09.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATTAGCTTTTGATGGC
Amino acid length 381
Molecular weight 41.5
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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