| Gene name |
SPBC30B4.08 |
| Gene ID |
18/A07 |
| Gene synonyms/obsolete |
|
| Gene product |
KapD family
exonuclease; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1142 |
| ORF length (spliced) |
834 |
| Entry clone length |
1142 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
866T:C / 1108C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC30B4.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGTCGCCAGTGCAGAT |
| Rev primer name |
SPBC30B4.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCTTGCGAAATATGGCGGG |
| Amino acid length |
277 |
| Molecular weight |
32.2 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
Confocal |