Gene name |
SPBP4H10.05c |
Gene ID |
17/H11 |
Gene synonyms/obsolete |
spe2 |
Gene product |
S-adenosylmethionine
decarboxylase proenzyme; involved in spermidine and spermine
biosynthesis; required for growth in the absence of
spermidine |
Entry clone |
Cloned |
ORF length (unspliced) |
1137 |
ORF length (spliced) |
|
Entry clone length |
1137 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
462A:G / 719T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBP4H10.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCCTACCCTTGTTGT |
Rev primer name |
SPBP4H10.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACTGCAGTAAAGCTGGCA |
Amino acid length |
378 |
Molecular weight |
42.7 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLASLPRLLEI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |