Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC317.01
Gene ID 17/F05
Gene synonyms/obsolete
Gene product MADS-box transcription factor; SRF-type transcription factor; involved in cell wall biosynthesis; deletion mutant galactomannans deficient for pyruvylated galactose-beta-1, 3-galactose-alpha-1,2-pyruvate (Pv) (pers. comm. Robert Trimble)
Entry clone Cloned
ORF length (unspliced) 1119
ORF length (spliced)
Entry clone length 1119
No. of intron 0
Sequence status Finished
Sequence results 699T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC317.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGGCGCAAAAAAATCTC
Rev primer name SPBC317.01.Rv
Rev primer SEQ AGAAAGCTGGGTAGTATTTGACATCACTGAGT
Amino acid length 372
Molecular weight 41.5
Isoelectric point (calc.) 7.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQQLNTLSI
Localization (YFP) nucleus>>cytosol; nuclear envelope?
Comments for localization a lot of cytoplasmic dots and nuclear dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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