| Gene name |
SPAC328.08c |
| Gene ID |
17/A12 |
| Gene synonyms/obsolete |
|
| Gene product |
tubulin specific
chaperone; TBCC family; CARP domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1092 |
| ORF length (spliced) |
780 |
| Entry clone length |
1092 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
302T:C / 353G:A /
905T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC328.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGAAAAGTTCGTCGA |
| Rev primer name |
SPAC328.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCGTCAAAAAGGTCACTA |
| Amino acid length |
259 |
| Molecular weight |
30 |
| Isoelectric point (calc.) |
8.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|