| Gene name |
SPBC3H7.05c |
| Gene ID |
16/E12 |
| Gene synonyms/obsolete |
|
| Gene product |
sequence orphan;
hypothetical protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1074 |
| ORF length (spliced) |
|
| Entry clone length |
1074 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
12T:C / 422T:C /
580T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3H7.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATCCATTTTCTTTCGA |
| Rev primer name |
SPBC3H7.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATTTGCGAGCATGTTTTC |
| Amino acid length |
357 |
| Molecular weight |
41.7 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
7 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFAIIFVPLGI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |