Gene name |
SPCC11E10.03 |
Gene ID |
16/B10 |
Gene synonyms/obsolete |
|
Gene product |
predicted coiled-coil
region; involved in nuclear migration (sensu Fungi); involved
in control of astral microtubules during mitotic growth |
Entry clone |
Cloned |
ORF length (unspliced) |
1056 |
ORF length (spliced) |
|
Entry clone length |
1056 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
199A:T / 659T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC11E10.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGAAATGATTAATCT |
Rev primer name |
SPCC11E10.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTGCAATTTTAAAACGCGT |
Amino acid length |
351 |
Molecular weight |
41.2 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNKCMEKLEI |
Localization (YFP) |
cytoplasmic dots;
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |