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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC428.06c
Gene ID 16/A09
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; non-essential
Entry clone Cloned
ORF length (unspliced) 1051
ORF length (spliced) 729
Entry clone length 1051
No. of intron 4
Sequence status Finished
Sequence results 203G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC428.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGCAATTTGAAGAACA
Rev primer name SPBC428.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGGAATTTGGTTTTCCATCA
Amino acid length 242
Molecular weight 27.2
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization nuclear dots by over expression?
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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