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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC651.02
Gene ID 16/A07
Gene synonyms/obsolete
Gene product carbon-nitrogen hydrolase family
Entry clone Cloned
ORF length (unspliced) 1050
ORF length (spliced) 831
Entry clone length 1050
No. of intron 4
Sequence status Finished
Sequence results 591A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC651.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACATTGGCAGCAGTAGC
Rev primer name SPBC651.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAATGGTCGGGTATAAATCG
Amino acid length 276
Molecular weight 30.4
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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