Gene name |
SPAC6F6.02c |
Gene ID |
15/H08 |
Gene synonyms/obsolete |
pof5 |
Gene product |
F-box protein; FTH
domain |
Entry clone |
Cloned |
ORF length (unspliced) |
1047 |
ORF length (spliced) |
|
Entry clone length |
1047 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
850T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6F6.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGTCGTTTCCTCCTGA |
Rev primer name |
SPAC6F6.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTCACTGTTATAAATACC |
Amino acid length |
348 |
Molecular weight |
38.9 |
Isoelectric point (calc.) |
7.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEYIILPYLSI |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |