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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC61.04c
Gene ID 15/F10
Gene synonyms/obsolete
Gene product Rab GTPase binding; Yip1 family; 5 predicted transmembrane helices; involved in intracellular protein transport; involved in ER to Golgi transport; interacts physically with SPBC25H2.06c; complexed with SPBC25H2.06c
Entry clone Cloned
ORF length (unspliced) 1035
ORF length (spliced) 684
Entry clone length 1035
No. of intron 2
Sequence status Finished
Sequence results 247T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC61.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTACTACAACAACCC
Rev primer name SPCC61.04.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCGAAAACACGGTGATC
Amino acid length 227
Molecular weight 25.3
Isoelectric point (calc.) 6.9
Signal SEQ
No. of transmembrane domain 5
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Leica

Image information
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