| Gene name |
SPCC61.04c |
| Gene ID |
15/F10 |
| Gene synonyms/obsolete |
|
| Gene product |
Rab GTPase binding;
Yip1 family; 5 predicted transmembrane helices; involved in
intracellular protein transport; involved in ER to Golgi
transport; interacts physically with SPBC25H2.06c; complexed
with SPBC25H2.06c |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1035 |
| ORF length (spliced) |
684 |
| Entry clone length |
1035 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
247T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC61.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTACTACAACAACCC |
| Rev primer name |
SPCC61.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTCGAAAACACGGTGATC |
| Amino acid length |
227 |
| Molecular weight |
25.3 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
5 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |