Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC21E11.07
Gene ID 15/F05
Gene synonyms/obsolete SPAC2C4.01
Gene product CCR4-Not complex; glycine cleavage T-protein (aminomethyl transferase) domain
Entry clone Cloned#
ORF length (unspliced) 1033
ORF length (spliced) 978
Entry clone length 1033
No. of intron 1
Sequence status Finished
Sequence results 17T:A
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC21E11.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAGGTTCACCGTTAG
Rev primer name SPAC21E11.07.Rv
Rev primer SEQ AGAAAGCTGGGTATGAATTTTCACTTGGCGTA
Amino acid length 325
Molecular weight 36.8
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.