Gene name |
SPAC4G9.14 |
Gene ID |
15/F02 |
Gene synonyms/obsolete |
|
Gene product |
similar to Sp
SPAC3G6.05 (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
1032 |
ORF length (spliced) |
666 |
Entry clone length |
1032 |
No. of intron |
5 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4G9.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTCATAGCGAAATCAAT |
Rev primer name |
SPAC4G9.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACGTCTGCATTCGGGGAA |
Amino acid length |
221 |
Molecular weight |
25.4 |
Isoelectric point (calc.) |
9.8 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQPLLTLGL/LDQFIFAPLGI |
Localization (YFP) |
Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |