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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP35G2.04c
Gene ID 15/E04
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1027
ORF length (spliced) 750
Entry clone length 1027
No. of intron 2
Sequence status Finished
Sequence results 565A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP35G2.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATATCTAATAGCTCCAT
Rev primer name SPBP35G2.04.Rv
Rev primer SEQ AGAAAGCTGGGTACTGCTTCGCTATCCGACAA
Amino acid length 249
Molecular weight 29.2
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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