| Gene name |
SPCC777.05 |
| Gene ID |
15/E02 |
| Gene synonyms/obsolete |
gtr2 |
| Gene product |
small GTPase;
interacts physically with Gtr1p (C-terminal) (2-hybrid) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1026 |
| ORF length (spliced) |
945 |
| Entry clone length |
1026 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
128A:G / 275A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC777.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGCCTAGAAAGATTAT |
| Rev primer name |
SPCC777.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGTTCTAGGTGAGAAAATG |
| Amino acid length |
314 |
| Molecular weight |
35.5 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPTLENLLNI/LREMNQYLAL |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |