| Gene name |
SPCC1494.01 |
| Gene ID |
14/H03 |
| Gene synonyms/obsolete |
SPCC965.15 |
| Gene product |
Iron/ascorbate
oxidoreductase family; no apparent Sc ortholog; 2 OG-Fe(II)
oxygenase superfamily; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1007 |
| ORF length (spliced) |
966 |
| Entry clone length |
1007 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
446C:T / 618T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1494.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGATCCTTAGAAGTACC |
| Rev primer name |
SPCC1494.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCCACAATACCATCATTT |
| Amino acid length |
321 |
| Molecular weight |
36.4 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |