| Gene name |
SPAC16E8.17c |
| Gene ID |
14/E07 |
| Gene synonyms/obsolete |
|
| Gene product |
succinate-CoA ligase
(alpha subunit); involved in succinyl-CoA metabolism; involved
in tricarboxylic acid cycle; succinate-CoA ligase
activity |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
996 |
| ORF length (spliced) |
|
| Entry clone length |
996 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC16E8.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTCAAGACACAGACAAC |
| Rev primer name |
SPAC16E8.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTGCTTCAATTTGTTGAGC |
| Amino acid length |
331 |
| Molecular weight |
34.7 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |