Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H3.15c
Gene ID 14/A06
Gene synonyms/obsolete SPAC25H1.01c
Gene product hypothetical serine-rich protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 978
ORF length (spliced)
Entry clone length 978
No. of intron 0
Sequence status Finished
Sequence results 536C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H3.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTACCAACAACGTGC
Rev primer name SPAC23H3.15.Rv
Rev primer SEQ AGAAAGCTGGGTAATACCTTTCAGAGTGATGG
Amino acid length 325
Molecular weight 34.6
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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