Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F3.01
Gene ID 13/F01
Gene synonyms/obsolete SPAC323.09
Gene product involved in the synthesis of mannosylated sphingolipids
Entry clone Cloned
ORF length (unspliced) 960
ORF length (spliced)
Entry clone length 960
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2F3.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAAGGAAATGTCGCAA
Rev primer name SPAC2F3.01.Rv
Rev primer SEQ AGAAAGCTGGGTAGGGATATAGCTCCTGTATT
Amino acid length 319
Molecular weight 37.1
Isoelectric point (calc.) 9.4
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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