| Gene name |
SPBC2D10.20 |
| Gene ID |
13/E05 |
| Gene synonyms/obsolete |
ubc1 |
| Gene product |
ubiquitin conjugating
enzyme |
| Entry clone |
Cloned |
| ORF length (unspliced) |
956 |
| ORF length (spliced) |
654 |
| Entry clone length |
956 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
188T:C / 723A:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2D10.20.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGACAATCGTTCTCG |
| Rev primer name |
SPBC2D10.20.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGTAATAGCTGATCTAAA |
| Amino acid length |
217 |
| Molecular weight |
24.5 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |