Gene name |
SPAC6B12.09 |
Gene ID |
12/F10 |
Gene synonyms/obsolete |
|
Gene product |
tRNA m(1)G
methyltransferase; conserved protein; implicated in human
cancers |
Entry clone |
Cloned |
ORF length (unspliced) |
915 |
ORF length (spliced) |
|
Entry clone length |
915 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
17C:T / 546T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6B12.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAACAAAGACGCTTT |
Rev primer name |
SPAC6B12.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTTTCTTTTTCCAATTCA |
Amino acid length |
304 |
Molecular weight |
35.4 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
56 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |