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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC5H10.01
Gene ID 12/D12
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 906
ORF length (spliced)
Entry clone length 906
No. of intron 0
Sequence status Finished
Sequence results 584T:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC5H10.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTACTACTAGTATCGA
Rev primer name SPAC5H10.01.Rv
Rev primer SEQ AGAAAGCTGGGTAACAATTATGGGTTAAATCA
Amino acid length 301
Molecular weight 33.7
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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