| Gene name |
SPCC4B3.14 |
| Gene ID |
11/G11 |
| Gene synonyms/obsolete |
cwf20 |
| Gene product |
involved in mRNA
splicing; complexed with Cdc5p; no apparent orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
873 |
| ORF length (spliced) |
|
| Entry clone length |
873 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
162T:G / 399A:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC4B3.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCTGGTTTCATATCC |
| Rev primer name |
SPCC4B3.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAACCCATACTTTTGAGAA |
| Amino acid length |
290 |
| Molecular weight |
32.5 |
| Isoelectric point (calc.) |
9.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |