Gene name |
SPCP31B10.01 |
Gene ID |
11/G04 |
Gene synonyms/obsolete |
cwf23;
SPCC10H11.02 |
Gene product |
DNAJ domain protein;
complexed with Cdc5p |
Entry clone |
Cloned |
ORF length (unspliced) |
870 |
ORF length (spliced) |
|
Entry clone length |
870 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
52G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCP31B10.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATCTGAAGGTGATTC |
Rev primer name |
SPCP31B10.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCGTTCATTGTAGAAGTG |
Amino acid length |
289 |
Molecular weight |
34.5 |
Isoelectric point (calc.) |
9.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |