Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1687.02
Gene ID 11/F05
Gene synonyms/obsolete
Gene product CAAX prenyl protease; involved in the proteolytic maturation of farnesylated proteins; peptidase family U48
Entry clone Cloned
ORF length (unspliced) 867
ORF length (spliced) 816
Entry clone length 867
No. of intron 1
Sequence status Finished
Sequence results 474T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1687.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGAGTTTATTTAATTTC
Rev primer name SPAC1687.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTTAATGGCACCAGTCTT
Amino acid length 271
Molecular weight 31
Isoelectric point (calc.) 8.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 5
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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