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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3H7.12
Gene ID 11/E09
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned#
ORF length (unspliced) 864
ORF length (spliced)
Entry clone length 864
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC3H7.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAAACGGAAATTGATGC
Rev primer name SPBC3H7.12.Rv
Rev primer SEQ AGAAAGCTGGGTATTTGGCACCATATAAAGAA
Amino acid length 287
Molecular weight 32
Isoelectric point (calc.) 8.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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