| Gene name |
SPAPJ698.02c |
| Gene ID |
11/E07 |
| Gene synonyms/obsolete |
rps002; rps0-2;
rpsa-2 |
| Gene product |
40S ribosomal protein
S0B |
| Entry clone |
Cloned |
| ORF length (unspliced) |
864 |
| ORF length (spliced) |
|
| Entry clone length |
864 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
check |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPJ698.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGAATCCATAGCCCG |
| Rev primer name |
SPAPJ698.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCCCATTCAGAAGGGGCA |
| Amino acid length |
287 |
| Molecular weight |
31.4 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
Leica |