| Gene name |
SPCC825.03c |
| Gene ID |
11/C10 |
| Gene synonyms/obsolete |
psy1 |
| Gene product |
SNARE; essential;
involved in secretory pathway; transcription enhanced during
sporulation; interacts genetically with spo3 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
855 |
| ORF length (spliced) |
|
| Entry clone length |
855 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
588A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC825.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATAAAGCAAACGATTA |
| Rev primer name |
SPCC825.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGTCTATTGCCAAGAACA |
| Amino acid length |
284 |
| Molecular weight |
32.5 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |