| Gene name |
SPAC29B12.06c |
| Gene ID |
11/B12 |
| Gene synonyms/obsolete |
rcd1 |
| Gene product |
RNA-binding protein;
involved in nitrogen starvation-induced sexual development
(required); negatively regulates differentiation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
852 |
| ORF length (spliced) |
|
| Entry clone length |
852 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
271T:C / 730T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC29B12.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAACTTGGAATCCCC |
| Rev primer name |
SPAC29B12.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTGATTAACAACGGCGACG |
| Amino acid length |
283 |
| Molecular weight |
32.2 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSTEIIPLCL/LKHVIRCYLRL |
| Localization (YFP) |
nucleus>cytosolG
nuclear dots; cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |