Gene name |
SPAPJ735.01c |
Gene ID |
10/D05 |
Gene synonyms/obsolete |
cwf16; SPAC9.13c |
Gene product |
eukaryotic conserved
protein; involved in mRNA splicing; interacts physically with
Cdc5p; interacts with other cwf proteins; involved in cell
cycle control |
Entry clone |
Cloned |
ORF length (unspliced) |
813 |
ORF length (spliced) |
|
Entry clone length |
813 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAPJ735.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGAACGAAAGGTACT |
Rev primer name |
SPAPJ735.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACCAAAACCTTTTTCTTC |
Amino acid length |
270 |
Molecular weight |
31.6 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB; nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |