| Gene name |
SPBC13G1.12 |
| Gene ID |
10/C05 |
| Gene synonyms/obsolete |
|
| Gene product |
SNF7 family; involved
in intracellular protein transport; involved in
protein-vacuolar targeting; class E vps |
| Entry clone |
Cloned |
| ORF length (unspliced) |
807 |
| ORF length (spliced) |
537 |
| Entry clone length |
807 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC13G1.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCACTATCAATGAATTT |
| Rev primer name |
SPBC13G1.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGACCTTAAAGCACGTAAA |
| Amino acid length |
178 |
| Molecular weight |
19.4 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|