Gene name |
SPAC186.05c |
Gene ID |
09/H08 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
UPF0016 family; similar to Sp SPAC17G8.08C |
Entry clone |
Cloned |
ORF length (unspliced) |
789 |
ORF length (spliced) |
|
Entry clone length |
789 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC186.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTAATGTTGAAAGTCC |
Rev primer name |
SPAC186.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAGCCTTGAAAAATGTAC |
Amino acid length |
262 |
Molecular weight |
29.2 |
Isoelectric point (calc.) |
9.5 |
Signal SEQ |
|
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |